SARS-CoV-2/COVID-19 Reagents

The TCF is currently producing reagents to study human T cell responses to SARS-CoV-2. These reagents are based on published work* as well as communication with the Sette lab and should be available as monomers or tetramers labeled with our usual selection of fluorochromes. Epitopes were previously identified in SARS and are 100% conserved in SARS-CoV-2. They are known to bind the MHC alleles we supply them with, but they have not been validated in a SARS-CoV-2 immune response.

These materials can be requested using the premade request forms below. You can use our standard class I and class II forms for custom SARS-CoV-2 reagents not listed here. Additional reagents may be added to or removed from the list depending on demand and validation. At this time, we can only supply 1 unit of quantity per reagent unless very strong justification for needing more can be supplied. These reagents will generally be offered at approximately 100µg per unit quantity.

* Grifoni A, Sidney J, Zhang Y, Scheuermann RH, Peters B, and Sette A. (2020) A Sequence Homology and Bioinformatic Approach Can Predict Candidate Targets for Immune Responses to SARS-CoV-2. Cell Host Microbe. PMID: 32183941.

 

Class I Premade SARS-CoV-2

Allele Description Peptide Sequence Order Options Info
H-2K(b) SARS-CoV-2 S 433-441 GNYNYLYRL
*
H-2K(b) SARS-CoV-2 S 525-532 VNFNFNGL
*
H-2K(b) SARS-CoV-2 S 435-443 YNYLYRLF
*
H-2K(d) SARS-CoV-2 S 365-373 CYGVSPTKL
*
HLA-A*02:01 SARS-CoV-2 S 958-996 ALNTLVKQL
HLA-A*02:01 SARS-CoV-2 N 138-146 ALNTPKDHI
HLA-A*02:01 SARS-CoV-2 S 1220-1228 FIAGLIAIV
HLA-A*02:01 SARS-CoV-2 N 315-324 GMSRIGMEV
HLA-A*02:01 SARS-CoV-2 N 350-358 ILLNKHIDA
HLA-A*02:01 SARS-CoV-2 S 996-1004 LITGRLQSL
HLA-A*02:01 SARS-CoV-2 N 222-230 LLLDRLNQL
HLA-A*02:01 SARS-CoV-2 N 159-167 LQLPQGTTL
HLA-A*02:01 SARS-CoV-2 S 1192-1200 NLNESLIDL
HLA-A*02:01 SARS-CoV-2 S 1185-1193 RLNEVAKNL
HLA-A*02:01 SARS-CoV-2 S 1000-1008 RLQSLQTYV

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Class II Premade SARS-CoV-2

Allele Description Peptide Sequence Order Options Info
DRB1*01:01 SARS-CoV-2 S 873-888 GAALQIPFAMQMAYRF
DRB1*04:01 SARS-CoV-2 S 461-475 LKPFERDISTEIYQA
DRB1*04:01 SARS-CoV-2 S 939-955 QALNTLVKQLSSNFGAI
DRB1*04:01 SARS-CoV-2 S 993-1010 QLIRAAEIRASANLAATK