Custom

(ex. SIINFEKL)
(ex. chicken)
(ex. ovalbumin)
(ex. 257-264)

Each unit of quantity contains 200 µg of MHC protein.

 


*Special case for mouse β2m:  Please note, the NIH Tetramer Facility normally folds all tetramers with human beta-2-microglobulin regardless of the species of MHC allele. If you think you need mouse β2m, please read this FAQ.


 

The Tetramer Resource Committee (TRC) meets approximately every other Friday to review tetramer requests. Requests must be received before 12 noon EST three days before a scheduled TRC meeting in order to be considered. View TRC Schedule

Click here for technical support.

Disclaimer: Please note that the reagents listed on this page have been produced by the NIH Tetramer Facility with strict quality control (including gene sequencing, affinity purification, SDS PAGE analysis, and verification of biotinylation by immunoprecipitation), but many have not been confirmed to stain or activate specific T cells.

Premade Class I

The class II MHC tetramers listed below are available as biotinylated monomers or as tetramers made with the listed fluorophore options. Requesters will receive approximately 200 micrograms of protein per unit of quantity.

Please note that the reagents listed on this page have been produced by the NIH Tetramer Facility with strict quality control (including gene sequencing, affinity purification, SDS PAGE analysis, and verification of biotinylation by immunoprecipitation), but many have not been confirmed to stain or activate specific T cells.

Some reagents may require you to send peptide to us in order to conduct a custom synthesis via peptide exchange. Once your order is approved, an email approval notification will be issued, including details regarding any peptide that needs to be supplied.

For those interested in requesting Unbiotinylated Monomers, please be advised that the Unbiotinylated Monomer format is not actively kept in stock for most reagents, and may require months to produce. Please be certain that our much more commonly stocked Biotinylated Monomer format will not be sufficient for your experimental needs before selecting the Unbiotinylated Monomer format.

Allele Description Peptide Sequence Order Options Info
H-2D(b) SIV Gag 312-322 AAVKNWMTQTL
H-2D(b) Influenza A NP 366-374 ASNENMETM
H-2D(b) LCMV NP 396-404 FQPQNGQFI
H-2D(b) MCMV M45 985-993 HGIRNASFI
H-2D(b) LCMV gp 33-41 KAVYNFATC
H-2D(b) LCMV GP var C41M 33-41 KAVYNFATM
H-2D(b) SARS-CoV-2 N 219-227 LALLLLDRL
*
H-2D(b) HPV16 E7 49-57 RAHYNIVTF
H-2D(b) LCMV gp 276-286 SGVENPGGYCL
H-2D(b) Influenza A PA 224-233 SSLENFRAYV
H-2D(b) Mouse SPAS-1 244-252 STHVNHLHC
*
H-2D(d) HIV-1 gp160 311-320 RGPGRAFVTI
H-2D(q) rat Her2/neu 420-429 PDSLRDLSVF
H-2K(b) mouse H60 39-46 LTFNYRNL
H-2K(b) VSV NP 52-59 RGYVYQGL

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