Please check the pre-made list below before placing a custom request.
Peptide may need to be supplied for some reagents.
Please note, a human CLIP peptide-containing negative control reagent will be included with all orders.
Custom Class II Reagents - Check Premade List (Below) Before Using
Each unit of quantity contains 200 µg of MHC protein.
The Tetramer Resource Committee (TRC) meets approximately every other Friday to review tetramer requests. Requests must be received before 12 noon EST three days before a scheduled TRC meeting in order to be considered. View TRC Schedule
Click here for technical support.
Previously Made Class II
The class II MHC tetramers listed below are available as biotinylated monomers or as tetramers labeled with APC, PE, or BV421. Requesters will receive 200 micrograms of protein per unit of quantity. In addition, a human CLIP peptide-containing negative control reagent will be included with all orders per allele. (This control will automatically be included. You do not need to add it to your request.)
Please note that the reagents listed on this page have been produced by the NIH Tetramer Facility with strict quality control (including gene sequencing, affinity purification, SDS PAGE analysis, and verification of biotinylation by immunoprecipitation), but many have not been confirmed to stain or activate specific T cells.
Some reagents may require you to send peptide to us in order to conduct a custom synthesis via peptide exchange. Once your order is approved, an email approval notification will be issued, including details regarding any peptide that needs to be supplied.
| Allele | Description | Peptide Sequence | Order Options | Info |
|---|---|---|---|---|
| I-A(b) | T. gondii TGME49__012300 605-619 | AVEIHRPVPGTAPPS | | |
| I-A(b) | mouse PLP 10-21 | CLVGAPFASLVA | | |
| I-A(b) | house dust mite Derp1 217-227 | CQIYPPNVNKI | | |
| I-A(b) | mouse TRP1 var C22A 113-126 | CRPGWRGAAANQKI | | |
| I-A(b) | mouse TRP1 113-126 | CRPGWRGAACNQKI | | |
| I-A(b) | LCMV GP 66-77 | DIYKGVYQFKSV | | |
| I-A(b) | Bac flagellin 456-475 | DMATEMVKYSNANILSQAGQ | | |
| I-A(b) | mouse Ealpha variant 55-68 | EAWGALANKAVDKA | | |
| I-A(b) | mouse 2W1S | EAWGALANWAVDSA | | |
| I-A(b) | mouse IgG 289-307 | EEQFNSTFRSVSELPIMHQ | | |
| I-A(b) | human IgG 318-338 | EEQYNSTYRVVSVLTVLHQDW | | |
| I-A(b) | FMLV env 123-141 | EPLTSLTPRCNTAWNRLKL | | |
| I-A(b) | Mtb esxG 46-61 | ESSAAFQAAHARFVAA | | |
| I-A(b) | mouse I-Ealpha 3K | FEAQKAKANKAVD | | |
| I-A(b) | Mtb Ag85b 280-294 | FQDAYNAAGGHNAVF | |
Pages
Disclaimer: Please note that the reagents listed on this page have been produced by the NIH Tetramer Facility with strict quality control (including gene sequencing, affinity purification, SDS PAGE analysis, and verification of biotinylation by immunoprecipitation), but many have not been confirmed to stain or activate specific T cells.