Please check the pre-made list below before placing a custom request.
Peptide may need to be supplied for some reagents.
Please note, a human CLIP peptide-containing negative control reagent will be included with all orders.
Custom Class II Reagents - Check Premade List (Below) Before Using
Each unit of quantity contains 200 µg of MHC protein.
The Tetramer Resource Committee (TRC) meets approximately every other Friday to review tetramer requests. Requests must be received before 12 noon EST three days before a scheduled TRC meeting in order to be considered. View TRC Schedule
Click here for technical support.
Previously Made Class II
The class I MHC tetramers listed below are available as biotinylated monomers or as tetramers made with the listed fluorophore options. Requesters will receive approximately 200 micrograms of protein per unit of quantity.
Please note that the reagents listed on this page have been produced by the NIH Tetramer Facility with strict quality control (including gene sequencing, affinity purification, SDS PAGE analysis, and verification of biotinylation by immunoprecipitation), but many have not been confirmed to stain or activate specific T cells.
Some reagents may require you to send peptide to us in order to conduct a custom synthesis via peptide exchange. Once your order is approved, an email approval notification will be issued, including details regarding any peptide that needs to be supplied.
| Allele | Description | Peptide Sequence | Order Options | Info |
|---|---|---|---|---|
| I-A(b) | mouse IgG 289-307 | EEQFNSTFRSVSELPIMHQ | | |
| I-A(b) | human IgG 318-338 | EEQYNSTYRVVSVLTVLHQDW | | |
| I-A(b) | FMLV env 123-141 | EPLTSLTPRCNTAWNRLKL | | |
| I-A(b) | Mtb esxG 46-61 | ESSAAFQAAHARFVAA | | |
| I-A(b) | mouse I-Ealpha 3K | FEAQKAKANKAVD | | |
| I-A(b) | Mtb Ag85b 280-294 | FQDAYNAAGGHNAVF | | |
| I-A(b) | M. avium Ag85b 280-294 | FQDAYNGAGGHNAVF | | |
| I-A(b) | Vaccinia B5R 46-60 | FTCDQGYHSSDPNAV | | |
| I-A(b) | GDVFVIREPFISCSH | | ||
| I-A(b) | Dengue virus 2 NS3 198-212 | GKTKRYLPAIVREAI | | |
| I-A(b) | Dengue virus 2 NS2B 108-122 | GLFPVSLPITAAAWY | | |
| I-A(b) | MPyV VP1 221-235 | GMYPVEIWHPDPAKN | | |
| I-A(b) | H. hep HH1713 230-244 | GNAYISVLAHYGKNG | | |
| I-A(b) | mouse MOG 38-49 var S42P | GWYRPPFSRVVH | | |
| I-A(b) | mouse MOG 38-49 var V47A | GWYRSPFSRAVH | |
Pages
Disclaimer: Please note that the reagents listed on this page have been produced by the NIH Tetramer Facility with strict quality control (including gene sequencing, affinity purification, SDS PAGE analysis, and verification of biotinylation by immunoprecipitation), but many have not been confirmed to stain or activate specific T cells.