Custom Class II

Please check the pre-made list below before placing a custom request..

 

Peptide may need to be supplied for some reagents.

 

Please note, a human CLIP peptide-containing negative control reagent will be included with all orders.

 

Each unit of quantity contains 200 µg of MHC protein.

 

The Tetramer Resource Committee (TRC) meets approximately every other Friday to review tetramer requests. Requests must be received before 12 noon EST three days before a scheduled TRC meeting in order to be considered. View TRC Schedule

Click here for technical support.

(ex. SIINFEKL)
(ex. chicken)
(APC or PE recommended for class II)
(ex. ovalbumin)
(ex. 66-77)
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Disclaimer: Please note that the reagents listed on this page have been produced by the NIH Tetramer Facility with strict quality control (including gene sequencing, affinity purification, SDS PAGE analysis, and verification of biotinylation by immunoprecipitation), but many have not been confirmed to stain or activate specific T cells.

PREVIOUSLY MADE CLASS II

The class II MHC tetramers listed below are available as biotinylated monomers or as tetramers made with the listed fluorophore options. Requesters will receive approximately 200 micrograms of protein per unit of quantity. For best results with class II tetramers, we strongly recommend APC or PE over other options.

Please note that the reagents listed on this page have been produced by the NIH Tetramer Facility with strict quality control (including gene sequencing, affinity purification, SDS PAGE analysis, and verification of biotinylation by immunoprecipitation), but many have not been confirmed to stain or activate specific T cells.

Some reagents may require you to send peptide to us in order to conduct a custom synthesis via peptide exchange. Once your order is approved, an email approval notification will be issued, including details regarding any peptide that needs to be supplied.

Allele Description Peptide Sequence Order Options Info
I-Ab chicken ova 328-337 HAAHAEINEA
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I-Ab MCMV m25 409-423 NHLYETPISATAMVI
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I-Ab mouse Ealpha 52-68 ASFEAQGALANIAVDKA
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I-Ab chicken ova 325-335 QAVHAAHAEIN
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I-Ab mouse IgG 167-186 PAVLQSDLYTLSSSVTVPSS
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I-Ab Mtb ESAT6 4-17 QQWNFAGIEAAASA
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I-Ab mouse Ealpha var I63K 52-68 ASFEAQGALANKAVDKA
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I-Ab synthetic epitope PADRE AKFVAAWTLKAA
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I-Ab human IgG 167-187 PAVLQSSGLYSLSSVVTVPSS
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I-Ab mouse IgG 289-307 EEQFNSTFRSVSELPIMHQ
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I-Ab mouse PLP 10-21 CLVGAPFASLVA
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I-Ab HSV-1 gD 290-302 IPPNWHIPSIQDA
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I-Ab Y. pestis F1CA 67-84 LTLGGYKTGTTSTSVNFT
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I-Ab Mtb RplJ 140-164 KAAGLFNAPASQLAR
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I-Ab T. gondii TGME49__012300 605-619 AVEIHRPVPGTAPPS
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