Product Type
Class II MHC
Description
This reagent recognizes a register of the ova 323-339 peptide that is present in wild-type CD4 T cell responses, but it does not bind to OT-II TCR transgenic T cells. You should request I-A(b) HAAHAEINEA or AAHAEINEA tetramers to stain OT-II cells.
Protein
I-Ab
Host Species
Mouse
Antigen Species
chicken
Antigen Molecule
ovalbumin
Peptide Sequence
QAVHAAHAEIN
Sequence Position
325-335
NCBI Blast Link

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PREVIOUSLY MADE CLASS II

The class II MHC tetramer listed below is available as biotinylated monomers or as tetramers made with the listed fluorophore options. Requesters will receive approximately 200 micrograms of protein per unit of quantity. For best results with class II tetramers, we strongly recommend APC or PE over other options.

Please note that this reagent has been produced by the NIH Tetramer Facility with strict quality control (including gene sequencing, affinity purification, SDS PAGE analysis, and verification of biotinylation by immunoprecipitation), but may have not been confirmed to stain or activate specific T cells.

Some reagents may require you to send peptide to us in order to conduct a custom synthesis via peptide exchange. Once your order is approved, an email approval notification will be issued, including details regarding any peptide that needs to be supplied.

Allele Description Peptide Sequence Order Options
I-Ab chicken ova 325-335 QAVHAAHAEIN
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