The TCF is currently producing reagents to study human T cell responses to SARS-CoV-2. These reagents are based on published work* as well as communication with the Sette lab and should be available by late May 2020 as monomers or tetramers labeled with our usual selection of fluorochromes. Epitopes were previously identified in SARS and are 100% conserved in SARS-CoV-2. They are known to bind the MHC alleles we supply them with, but they have not been validated in a SARS-CoV-2 immune response.

Class I

HLA-A*02:01   S 1185-1193    RLNEVAKNL
HLA-A*02:01   S 1220-1228    FIAGLIAIV
HLA-A*02:01   S 996-1004      LITGRLQSL
HLA-A*02:01   S 976-984        VLNDILSRL
HLA-A*02:01   N 222-230       LLLDRLNQL
HLA-A*02:01   S 958-996        ALNTLVKQL
HLA-A*02:01   S 1192-1200    NLNESLIDL
HLA-B*08:01   M 148-156      HLRIAGHHL
HLA-B*40:01   N 322-331       MEVTPSGTWL

Class II

DRB1*04:01    S 461-475        LKPFERDISTEIYQA
DRB1*04:01    S 993-1010      QLIRAAEIRASANLAATK

We have ordered peptides to produce these reagents and hope to begin shipping the tetramers (or monomeric MHCp) reagents sometime in May 2020. These materials can be requested using the process described on the front page of our website, along with custom requests for SARS-CoV-2 reagents not listed here. Additional reagents may be added to the list above depending on demand and validation. 

* Grifoni A, Sidney J, Zhang Y, Scheuermann RH, Peters B, and Sette A. (2020) A Sequence Homology and Bioinformatic Approach Can Predict Candidate Targets for Immune Responses to SARS-CoV-2. Cell Host Microbe. PMID: 32183941.