The TCF is currently producing reagents to study human T cell responses to SARS-CoV-2. These reagents are based on published work* as well as communication with the Sette lab and should be available by late May 2020 as monomers or tetramers labeled with our usual selection of fluorochromes. Epitopes were previously identified in SARS and are 100% conserved in SARS-CoV-2. They are known to bind the MHC alleles we supply them with, but they have not been validated in a SARS-CoV-2 immune response.
HLA-A*02:01 S 1185-1193 RLNEVAKNL
HLA-A*02:01 S 1220-1228 FIAGLIAIV
HLA-A*02:01 S 996-1004 LITGRLQSL
HLA-A*02:01 S 976-984 VLNDILSRL
HLA-A*02:01 N 222-230 LLLDRLNQL
HLA-A*02:01 S 958-996 ALNTLVKQL
HLA-A*02:01 S 1192-1200 NLNESLIDL
HLA-B*08:01 M 148-156 HLRIAGHHL
HLA-B*40:01 N 322-331 MEVTPSGTWL
DRB1*04:01 S 461-475 LKPFERDISTEIYQA
DRB1*04:01 S 993-1010 QLIRAAEIRASANLAATK
We have ordered peptides to produce these reagents and hope to begin shipping the tetramers (or monomeric MHCp) reagents sometime in May 2020. These materials can be requested using the process described on the front page of our website, along with custom requests for SARS-CoV-2 reagents not listed here. Additional reagents may be added to the list above depending on demand and validation.
* Grifoni A, Sidney J, Zhang Y, Scheuermann RH, Peters B, and Sette A. (2020) A Sequence Homology and Bioinformatic Approach Can Predict Candidate Targets for Immune Responses to SARS-CoV-2. Cell Host Microbe. PMID: 32183941.