After initiation of anti-retroviral therapy (ART), HIV viral load and frequencies of HIV-epitope-specific immune responses decrease. A diverse virus-specific T cell receptor (TCR) repertoire allows the host to respond to viral epitope diversity, but the effect of antigen reduction as a result of ART on the TCR repertoire of epitope-specific CD8+ T cell populations has not been well defined.We determined the TCR repertoires of 14 HIV-specific CD8+ T cell responses from 8 HIV+ individuals before and after initiation of ART. We used multiparameter flow cytometry to measure the distribution of memory T cell subsets and the surface expression of PD-1 on T cell populations and T cell clonotypes within epitope-specific responses from these individuals. Post-ART, we noted decreases in the frequencies of circulating epitope specific T-cells (p=0.02), decreases in the number of T-cell clonotypes found within epitope-specific T cell receptor repertoires (p=0.024), and an overall reduction in the amino acid diversity within these responses (p<0.0001). Despite this narrowing of the T cell response to HIV, the overall hierarchy of dominant T cell receptor clonotypes remained stable compared to pre-ART. CD8+ T cells underwent redistributions in memory phenotypes and a reduction in CD38 and PD-1 expression post-ART. Despite extensive remodeling at a structural and phenotypic level, PD-1 was expressed at higher levels on dominant clonotypes within epitope-specific responses before and after initiation of ART. These data suggest that antigen burden may maintain TCR diversity, and that dominant clonotypes are sensitive to antigen even after dramatic reductions after initiation of ART