Placing a Request
Your lab must have established a Principal Investigator account before you can place a request on our site. Please see details about setting up a PI account here.
There are two main ways by which you can request reagents from our facility:
- If requesting refills: Look at your order history, which can be accessed by clicking on your username icon on the top right of the page. Find the order for which you wish to request a refill, and click the ‘Add Items to Cart’ button. If there are some items in that order you do not wish to reorder, you may delete them out of your shopping cart individually.
- If placing a new request: Use one of the 5 different request forms, depending on the type of reagent you want.
Class I: While we do offer some common mouse class I reagents from a pre-made common stock, most class I reagent requests require custom synthesis. This form will allow you to place a request for all classical class I and peptide binding nonclassical class I reagents, such as HLA*E and Qa-1 reagents.
Class II: This form is broken into two parts: custom requests, found at the top of the form, and pre-made requests. We have over 200 pre-made reagents available from our facility. You can use the search filters to see if the reagent is available from our pre-made list. If using the premade list, don’t forget to enter the quantity and form requested. Furthermore, requesting a premade reagent automatically adds a control reagent to your cart; you do not need to request the control yourself.
CD1: We offer pre-made CD1 reagents from several species, either empty or loaded with PBS57 or OCH ligands. Requesting a loaded CD1 reagent will automatically add an unloaded control to your cart.
CD1d Ligands: We offer 3 different ‘ligand only’ reagents, which can be requested using this form.
Other: This form has a custom request section and a pre-made section as well. It is used for the request of non-ligand binding MHC or other proteins.
Our site now has shopping cart functionality. As requests are submitted, they are added to your shopping cart to await checkout. You can even log out and come back to this later; the shopping cart will keep your requests around until you are ready to check out.
Once you have submitted all your reagent requests, you are ready to check out. The checkout process involves several steps, each presented on a separate page.
1. Shipping information - this page allows you to select from one of your established shipping profiles, or to create a new shipping profile.
2. Supporting Documentation - this page is where you’ll enter the details about the research you’re doing, and why you need this particular reagent. All fields are currently required, so if a particular field doesn’t apply to you, you can enter ‘n/a’ or ‘none’. Please note that our new site design only requires you to enter this supporting information once per order during the checkout process. Therefore, if your lab is working on multiple projects, it would make most sense to submit separate orders for the different projects, so that each order reflects the relevant abstract details of that research project. If you choose to put reagent requests from multiple projects into the same order, you must enter appropriate information regarding each project into the relevant sections.
3. Staff - This page is where you will enter the staff to be associated with this order, including all lab/primary contacts, staff members using the reagent, and collaborators/subcontractors that will be using the reagent. You can select from a checklist of all existing sub-accounts, or you can create a new sub-account at this step as required. Any sub-accounts created will then become available in the checklist for association on the present and all future orders placed.
The tetramer request follow-up process is managed by two entities:
1. The NIAID/NIH Project Officer and the Tetramer Resource Committee (TRC) at the NIH in Bethesda, MD
2. The Facility Manager at the NIH Tetramer Facility at Emory University in Atlanta, GA
The follow-up process can be broken down into three steps.
Step 1: All reagent requests, whether new or for refills, should be placed through our website according to the instructions above. Our site now allows a single order to contain as many reagent requests as required. You may, however, be asked to prioritize items in especially large orders, in order to ensure equal access to our services for all clients.
These requests are automatically sent to the NIH TRC for review. It is important that the requester complete all fields of the request form, even for refill requests. Incomplete request forms will be held for consideration until the required information is provided.
Requests will be reviewed in a confidential manner by the NIH TRC comprised of NIH staff. The NIH TRC meets approximately every two weeks to review, approve and prioritize applications. All requests received by 12 pm Eastern Time on the day prior to the meeting are reviewed at the TRC meeting on the following day. All requests received after 12 pm are reviewed at the next meeting.
All clients are required to have a current Materials Transfer Agreement between a Principal Invesigator at their respective Institution and the NIH Tetramer Core Facility. Please note that the MTA policy is different for non-profit clients, for-profit clients, and non-profit clients with funding from for-profit sources.
Non-profit clients must complete our standardized MTA, which can be downloaded from our website. This registration only needs to be done once, as long as the PI remains at the same institution ( http://tetramer.yerkes.emory.edu/support/mta).
For-profit clients are required to complete a separate MTA which is individually drafted by NIH for the specific client and reagents being requested. This must be completed for each specific reagent that is requested from our facility. Please contact our Project Officer, Alison Deckhut-Augustine ( firstname.lastname@example.org ) to begin the process of drafting this specialized document. It is best to submit an order for the specified reagents first, as described below. This will provide us with the specific details of the request in order to begin drafting the document.
Non-profit clients which will be using our materials for research being funded by for-profit entities must complete both types of MTAs as described above.
Step 2: Requesters are informed of approval by Dale Long, NIH Tetramer Facility Manager, via email and given additional instructions for shipping peptides and/or transport containers to the NIH Tetramer Facility.
Step 3: The NIH Tetramer Facility Manager organizes reagent production and shipment to requesters. The Facility Manager may contact the requester to coordinate shipments or to discuss any issues related to production of the approved reagents.. Requesters may also contact the Facility Manager to receive a progress report on a particular order.
For custom-made class I reagents and some human class II reagents, requesters are required to submit the necessary epitope peptides to the NIH Tetramer Facility within four months of request approval. Approved requests that do not meet this deadline will be inactivated, and the requester will have to submit a new request online. Epitope peptide submissions should normally be 10 mg (for class I tetramers) or 2 mg (for custom made class II) of lyophilized, >80% pure peptide. Note that there are some exceptions; for example, the class I alleles HLA-B*57xx are particularly difficult to fold, and so we ask for a minimum of 25mg in this case.
Please note that for custom-made class I reagents it takes at least one month minimum from the time the NIH Tetramer Facility receives the peptide to the time the reagent is ready to be shipped. Custom class II tetramer synthesis may take three to four months, depending on the MHC alelle requested (for those alleles that require tethered consructs). The time frame for receiving a reagent can vary depending upon the timing of the TRC meeting, the workload at the Tetramer Facility, technical issues related to actual tetramer production, and shipping delays.