Custom Class II Reagents - Check Premade List (Below) Before Using

(ex. DIYKGVYQFKSV)
(ex. LCMV)
(ex. glycoprotein)
(ex. 66-77)

Please check the pre-made list below before placing a custom request.

Peptide may need to be supplied for some reagents.

Please note, a human CLIP peptide-containing negative control reagent will be included with all orders.

Each unit of quantity contains 200 µg of MHC protein.

The Tetramer Resource Committee (TRC) meets approximately every other Friday to review tetramer requests. Requests must be received before 12 noon EST on the Thursday before a scheduled TRC meeting in order to be considered. View TRC Schedule

Click here for technical support.

Previously Made Class II

The class II MHC tetramers listed below are available as biotinylated monomers or as tetramers labeled with APC, PE, or BV421. Requesters will receive 200 micrograms of protein per unit of quantity. In addition, a human CLIP peptide-containing negative control reagent will be included with all orders per allele. (This control will automatically be included. You do not need to add it to your request.)

Please note that the reagents listed on this page have been produced by the NIH Tetramer Facility with strict quality control (including gene sequencing, affinity purification, SDS PAGE analysis, and verification of biotinylation by immunoprecipitation), but many have not been confirmed to stain or activate specific T cells.

Some reagents may require you to send peptide to us in order to conduct a custom synthesis via peptide exchange. Once your order is approved, an email approval notification will be issued, including details regarding any peptide that needs to be supplied.

Allele Description Peptide Sequence Order Options Info
DPB1*04:01 C. tetani TT 948-968 FNNFTVSFWLRVPKVSASHLE
DPB1*04:01 human MAGE3 243-258 KKLLTQHFVQENYLEY
DPB1*04:01 human oxytocinase 272-284 KKYFAATQFEPLA
DPB1*04:01 human CLIP 87-101 PVSKMRMATPLLMQA
DPB1*04:01 human CTAG1 157-170 SLLMWITQCFLPVF
DPB1*04:01 HIV env 31-45 TEKLWVTVYYGVPVW
DQB1*03:02/DQA1*03:01 human CLIP 87-101 PVSKMRMATPLLMQA
DQB1*03:02/DQA1*03:01 human FcR2 104-119 QDLELSWNLNGLQADL
DQB1*05:01/DQA1*01:01 HIV gag 293-303 FRDYVDRFYKT
DQB1*05:01/DQA1*01:01 human CLIP 87-101 PVSKMRMATPLLMQA
DQB1*05:01/DQA1*01:01 human FcR2 104-122 SQDLELSWNLNGLQADLSS
DRB1*01:01 human CLIP 87-101 PVSKMRMATPLLMQA
DRB1*03:01 human SS-B/La 313-330 EALKKIIEDQQESLNKWK
*
DRB1*03:01 human Ro60 241-265 ELEVIHLIEEHRLVREHLLTNHLKS
*
DRB1*03:01 human S-arrestin 106-117 ERRGIALDGKIK
*

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Disclaimer: Please note that the reagents listed on this page have been produced by the NIH Tetramer Facility with strict quality control (including gene sequencing, affinity purification, SDS PAGE analysis, and verification of biotinylation by immunoprecipitation), but many have not been confirmed to stain or activate specific T cells.