Custom Class II Reagents - Check Premade List (Below) Before Using

(ex. DIYKGVYQFKSV)
(ex. LCMV)
(ex. glycoprotein)
(ex. 66-77)

Please check the pre-made list below before placing a custom request.

Peptide may need to be supplied for some reagents.

Please note, a human CLIP peptide-containing negative control reagent will be included with all orders.

Each unit of quantity contains 200 µg of MHC protein.

The Tetramer Resource Committee (TRC) meets approximately every other Friday to review tetramer requests. Requests must be received before 12 noon EST three days before a scheduled TRC meeting in order to be considered. View TRC Schedule

Click here for technical support.

Previously Made Class II

The class I MHC tetramers listed below are available as biotinylated monomers or as tetramers made with the listed fluorophore options. Requesters will receive approximately 200 micrograms of protein per unit of quantity.

Please note that the reagents listed on this page have been produced by the NIH Tetramer Facility with strict quality control (including gene sequencing, affinity purification, SDS PAGE analysis, and verification of biotinylation by immunoprecipitation), but many have not been confirmed to stain or activate specific T cells.

Some reagents may require you to send peptide to us in order to conduct a custom synthesis via peptide exchange. Once your order is approved, an email approval notification will be issued, including details regarding any peptide that needs to be supplied.

Allelesort descending Description Peptide Sequence Order Options Info
DRB5*01:01 human CLIP 87-101 PVSKMRMATPLLMQA
I-A(b) WNV E 641-655 PVGRLVTVNPFVSVA
I-A(b) chicken Ova 329-337 AAHAEINEA
I-A(b) mouse 2W1S EAWGALANWAVDSA
I-A(b) Vaccinia H3L 269-283 PGVMYAFTTPLISFF
I-A(b) MNV1 Capsid 496-513 YIAVSYSGSGPLTFPTDG
I-A(b) S. enterica FliC 428-442 VQNRFNSAITNLGNT
I-A(b) Dengue virus 2 NS4b 2339-2353 IGCYSQVNPITLTAA
I-A(b) LCMV GP 66-77 DIYKGVYQFKSV
I-A(b) E. coli bgal 721-739 AENLSVTLPAASHAIPHLT
I-A(b) human fVIII 2210-2229 TASSYFTNMFATWSPSKARL
I-A(b) Vaccinia B5R 46-60 FTCDQGYHSSDPNAV
I-A(b) SV40 T Ag 526-540 VTMNEYSVPKTLQAR
I-A(b) mouse MOG 38-49 var S42P GWYRPPFSRVVH
I-A(b) synthetic eGFP 81-95 HDFFKSAMPEGYVQE

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Disclaimer: Please note that the reagents listed on this page have been produced by the NIH Tetramer Facility with strict quality control (including gene sequencing, affinity purification, SDS PAGE analysis, and verification of biotinylation by immunoprecipitation), but many have not been confirmed to stain or activate specific T cells.