Custom Class II Reagents - Check Premade List (Below) Before Using

(ex. DIYKGVYQFKSV)
(ex. LCMV)
(ex. glycoprotein)
(ex. 66-77)

Please check the pre-made list below before placing a custom request.

Peptide may need to be supplied for some reagents.

Please note, a human CLIP peptide-containing negative control reagent will be included with all orders.

Each unit of quantity contains 200 µg of MHC protein.

The Tetramer Resource Committee (TRC) meets approximately every other Friday to review tetramer requests. Requests must be received before 12 noon EST on the Thursday before a scheduled TRC meeting in order to be considered. View TRC Schedule

Click here for technical support.

Previously Made Class II

The class II MHC tetramers listed below are available as biotinylated monomers or as tetramers labeled with APC, PE, or BV421. Requesters will receive 200 micrograms of protein per unit of quantity. In addition, a human CLIP peptide-containing negative control reagent will be included with all orders per allele. (This control will automatically be included. You do not need to add it to your request.)

Please note that the reagents listed on this page have been produced by the NIH Tetramer Facility with strict quality control (including gene sequencing, affinity purification, SDS PAGE analysis, and verification of biotinylation by immunoprecipitation), but many have not been confirmed to stain or activate specific T cells.

Some reagents may require you to send peptide to us in order to conduct a custom synthesis via peptide exchange. Once your order is approved, an email approval notification will be issued, including details regarding any peptide that needs to be supplied.

Allele Description Peptide Sequence Order Options Info
I-A(b) L. major GPC 335-351 NDAFGVMPPVARLTPEQ
I-A(b) Lm LLO 190-201 NEKYAQAYPNVS
I-A(b) MPyV LT 676-688 NEYLLPQTVWARF
I-A(b) SV40 T Ag 529-543 NEYSVPKTLQARFVK
I-A(b) MCMV m25 409-423 NHLYETPISATAMVI
I-A(b) RSV M 209-223 NKGAFKYIKPQSQFI
I-A(b) gHV68 M2 124-138 NSEPVYIQPISTRSL
I-A(b) RSV M2-1 26-39 NYFEWPPHALLVRQ
I-A(b) MNV1 NS1-2 46-63 NYMTPPEQEAQPGALAAL
I-A(b) human PSMA 231-245 PADYFAPGVKSYPDG
I-A(b) mouse IgG 167-186 PAVLQSDLYTLSSSVTVPSS
I-A(b) human IgG 167-187 PAVLQSSGLYSLSSVVTVPSS
I-A(b) mouse H-2K(d) 57-71 PEYWEEQTQRAKSD
I-A(b) Vaccinia H3L 269-283 PGVMYAFTTPLISFF
I-A(b) human IDH1 R132H 127-141 PIIIGHHAYGDQYRA

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Disclaimer: Please note that the reagents listed on this page have been produced by the NIH Tetramer Facility with strict quality control (including gene sequencing, affinity purification, SDS PAGE analysis, and verification of biotinylation by immunoprecipitation), but many have not been confirmed to stain or activate specific T cells.