Custom Class II Reagents - Check Premade List (Below) Before Using

Please check the pre-made list below before placing a custom request.

Peptide may need to be supplied for some reagents.

Please note, a human CLIP peptide-containing negative control reagent will be included with all orders.

Each unit of quantity contains 200 µg of MHC protein.

The Tetramer Resource Committee (TRC) meets approximately every other Friday to review tetramer requests. Requests must be received before 12 noon EST on the Thursday before a scheduled TRC meeting in order to be considered. View TRC Schedule

Click here for technical support.

Previously Made Class II

The class II MHC tetramers listed below are available as biotinylated monomers or as tetramers labeled with APC, PE, or BV421. Requesters will receive 200 micrograms of protein per unit of quantity. In addition, a human CLIP peptide-containing negative control reagent will be included with all orders per allele. (This control will automatically be included. You do not need to add it to your request.)

Please note that the reagents listed on this page have been produced by the NIH Tetramer Facility with strict quality control (including gene sequencing, affinity purification, SDS PAGE analysis, and verification of biotinylation by immunoprecipitation), but many have not been confirmed to stain or activate specific T cells.

Some reagents may require you to send peptide to us in order to conduct a custom synthesis via peptide exchange. Once your order is approved, an email approval notification will be issued, including details regarding any peptide that needs to be supplied.

Allele Description Peptide Sequence Order Options Info
I-A(b) MCMV m09 131-145 GYLYIYPSAGNSFDL
I-A(b) chicken Ova 328-337 HAAHAEINEA
I-A(b) L. major DLD 63-79 HATHLYHDAHANFAQYG
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I-A(b) Dengue virus 2 NS4b 2339-2353 IGCYSQVNPITLTAA
I-A(b) HSV-1 gD 290-302 IPPNWHIPSIQDA
I-A(b) Mtb RplJ 140-164 KAAGLFNAPASQLAR
I-A(b) C. trach Cta1 133-152 KGIDPQELWVWKKGMPNWEK
I-A(b) C. albicans ALS1 236-253 KGLNDWNYPVSSESFSYT
I-A(b) human ABPP 687-701 KLVFFAEDVGSNKGA
I-A(b) T. californica ACHRa 170-186 LGIWTYDGTKVSISPES
I-A(b) human fIX 318-329 LLELDEPLVLNS
I-A(b) MHV E2 358-372 LSSLLRYVQAESLSC
I-A(b) Y. pestis F1CA 67-84 LTLGGYKTGTTSTSVNFT
I-A(b) human IRBP 26-34 LVLDMAKVL
I-A(b) mouse tyrosinase 99-107 NCGNCKFGFGGPNCTEKRV
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Disclaimer: Please note that the reagents listed on this page have been produced by the NIH Tetramer Facility with strict quality control (including gene sequencing, affinity purification, SDS PAGE analysis, and verification of biotinylation by immunoprecipitation), but many have not been confirmed to stain or activate specific T cells.