Custom Class II Reagents - Check Premade List (Below) Before Using

Please check the pre-made list below before placing a custom request.

Peptide may need to be supplied for some reagents.

Please note, a human CLIP peptide-containing negative control reagent will be included with all orders.

Each unit of quantity contains 200 µg of MHC protein.

The Tetramer Resource Committee (TRC) meets approximately every other Friday to review tetramer requests. Requests must be received before 12 noon EST on the Thursday before a scheduled TRC meeting in order to be considered. View TRC Schedule

Click here for technical support.

Previously Made Class II

The class II MHC tetramers listed below are available as biotinylated monomers or as tetramers labeled with APC, PE, or BV421. Requesters will receive 200 micrograms of protein per unit of quantity. In addition, a human CLIP peptide-containing negative control reagent will be included with all orders per allele. (This control will automatically be included. You do not need to add it to your request.)

Please note that the reagents listed on this page have been produced by the NIH Tetramer Facility with strict quality control (including gene sequencing, affinity purification, SDS PAGE analysis, and verification of biotinylation by immunoprecipitation), but many have not been confirmed to stain or activate specific T cells.

Some reagents may require you to send peptide to us in order to conduct a custom synthesis via peptide exchange. Once your order is approved, an email approval notification will be issued, including details regarding any peptide that needs to be supplied.

Allele Description Peptide Sequence Order Options Info
DRB1*08:03 human CLIP 87-101 PVSKMRMATPLLMQA
DRB1*09:01 Mtb ESAT6 1-15 MTEQQWNFAGIEAAA
*
DRB1*09:01 human CLIP 87-101 PVSKMRMATPLLMQA
DRB1*14:01 human CLIP 87-101 PVSKMRMATPLLMQA
DRB1*14:03 Mtb ESAT6 73-87 ARTISEAGQAMASTE
*
DRB1*14:03 human CLIP 87-101 PVSKMRMATPLLMQA
DRB1*15:01 human CLIP 87-101 PVSKMRMATPLLMQA
DRB1*15:02 human CLIP 87-101 PVSKMRMATPLLMQA
DRB4*01:01 BORPE prn 41-58 IVKTGERQHGIHIQGSDP
*
DRB4*01:01 human CLIP 87-101 PVSKMRMATPLLMQA
DRB5*01:01 human CLIP 87-101 PVSKMRMATPLLMQA
I-A(b) chicken Ova 329-337 AAHAEINEA
I-A(b) E. coli bgal 721-739 AENLSVTLPAASHAIPHLT
I-A(b) synthetic epitope PADRE AKFVAAWTLKAA
I-A(b) mouse Ealpha 52-68 ASFEAQGALANIAVDKA

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Disclaimer: Please note that the reagents listed on this page have been produced by the NIH Tetramer Facility with strict quality control (including gene sequencing, affinity purification, SDS PAGE analysis, and verification of biotinylation by immunoprecipitation), but many have not been confirmed to stain or activate specific T cells.