Custom Class II

Please check the pre-made list below before placing a custom request..

 

Peptide may need to be supplied for some reagents.

 

Please note, a human CLIP peptide-containing negative control reagent will be included with all orders.

 

Each unit of quantity contains 200 µg of MHC protein.

 

The Tetramer Resource Committee (TRC) meets approximately every other Friday to review tetramer requests. Requests must be received before 12 noon EST three days before a scheduled TRC meeting in order to be considered. View TRC Schedule

Click here for technical support.

(ex. SIINFEKL)
(ex. chicken)
(APC or PE recommended for class II)
(ex. ovalbumin)
(ex. 66-77)
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Disclaimer: Please note that the reagents listed on this page have been produced by the NIH Tetramer Facility with strict quality control (including gene sequencing, affinity purification, SDS PAGE analysis, and verification of biotinylation by immunoprecipitation), but many have not been confirmed to stain or activate specific T cells.

PREVIOUSLY MADE CLASS II

The class II MHC tetramers listed below are available as biotinylated monomers or as tetramers made with the listed fluorophore options. Requesters will receive approximately 200 micrograms of protein per unit of quantity. For best results with class II tetramers, we strongly recommend APC or PE over other options.

Please note that the reagents listed on this page have been produced by the NIH Tetramer Facility with strict quality control (including gene sequencing, affinity purification, SDS PAGE analysis, and verification of biotinylation by immunoprecipitation), but many have not been confirmed to stain or activate specific T cells.

Some reagents may require you to send peptide to us in order to conduct a custom synthesis via peptide exchange. Once your order is approved, an email approval notification will be issued, including details regarding any peptide that needs to be supplied.

Allele Description Peptide Sequence Order Options Info
I-Ag7 mouse IAP 38-57 KCNTATCATQRLANFLVRSS
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I-Ag7 mouse GAD65 217-236 EYVTLKKMREIIGWPGGSGD
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I-Ag7 mouse GAD65 286-300 KKGAAALGIGTDSVI
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I-Ag7 BDC2.5 mimotope SRLGLWVRME
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I-Ag7 mouse MBP 74-85 SRPGLCHMYKDS
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I-Ag7 mimotope G7m GKKVATFVHAGYG
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I-Ag7 BDC2.5 mimotope AHHPIWARMDA
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I-Ag7 BDC2.5 mimotope RTRPLWVRME
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I-Ag7 mouse ProIns 47-64 SPGDLQTLALEVARQKRG
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I-Ag7 BDC15 mimotope AAAAVRPLWVRMEAA
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I-Ag7 chicken Ova 141-160 CARELINSWVESQTNGIIRN
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I-Ag7 mouse GAD65 260-285 PEVKEKGMAALPRLIAFTSE
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I-Ag7 mouse InsB 12-20 VEALYLVCG
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I-Ag7 rat CD23 216-231 DLKGRLVSIHSQKEQD
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I-Ag7 mouse ChgA 358-371 WSRMDQLAKELTAE
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