Custom Class II Reagents - Check Premade List (Below) Before Using

(ex. DIYKGVYQFKSV)
(ex. LCMV)
(ex. glycoprotein)
(ex. 66-77)

Please check the pre-made list below before placing a custom request.

Peptide may need to be supplied for some reagents.

Please note, a human CLIP peptide-containing negative control reagent will be included with all orders.

Each unit of quantity contains 200 µg of MHC protein.

The Tetramer Resource Committee (TRC) meets approximately every other Friday to review tetramer requests. Requests must be received before 12 noon EST three days before a scheduled TRC meeting in order to be considered. View TRC Schedule

Click here for technical support.

Previously Made Class II

The class II MHC tetramers listed below are available as biotinylated monomers or as tetramers labeled with APC, PE, or BV421. Requesters will receive 200 micrograms of protein per unit of quantity. In addition, a human CLIP peptide-containing negative control reagent will be included with all orders per allele. (This control will automatically be included. You do not need to add it to your request.)

Please note that the reagents listed on this page have been produced by the NIH Tetramer Facility with strict quality control (including gene sequencing, affinity purification, SDS PAGE analysis, and verification of biotinylation by immunoprecipitation), but many have not been confirmed to stain or activate specific T cells.

Some reagents may require you to send peptide to us in order to conduct a custom synthesis via peptide exchange. Once your order is approved, an email approval notification will be issued, including details regarding any peptide that needs to be supplied.

Allele Description Peptide Sequencesort ascending Order Options Info
I-A(b) S. enterica SseJ 330-339 YYETADAFKV
I-A(b) C. trachomatis PmpG 303-311 YVDPAAAGG
*
I-A(k) T. brucei VSG 237-251 YVDGYIQVTAAANSK
I-A(b) bac flagellin 464-472 YSNANILSQ
I-A(b) mouse ApoB100 3717-3725 YSFSIPVKV
I-A(g7) rat E25B 112-126 YQTIEENIKIFEEDA
I-A(b) MNV1 Capsid 496-513 YIAVSYSGSGPLTFPTDG
I-A(b) SARS NP 110-124 YFYYLGTGPEASLPY
I-A(b) RSV M2-1 27-37 YFEWPPHALLV
DRB1*03:01 human SS-B/La 46-63 WVPLEIMIKFNRLNRLTT
*
I-A(g7) mouse ChgA 358-371 WSRMDQLAKELTAE
DRB1*03:01 human survivin 10-24 WQPFLKDHRISTFKN
*
DRB1*07:01 HCV NS2 47-63 WPLLLLLLALPQRAYAQ
*
Patr-DRB5*01:02 HCV NS4b 50-64 WAKHMWNFISGIQYL
DRB1*03:01 HCV NS5b 66-81 VYYLTRDPTTPLARAA
*

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Disclaimer: Please note that the reagents listed on this page have been produced by the NIH Tetramer Facility with strict quality control (including gene sequencing, affinity purification, SDS PAGE analysis, and verification of biotinylation by immunoprecipitation), but many have not been confirmed to stain or activate specific T cells.