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PBS-57
PBS-57 is an analogue of a-galactosylceramide recently developed
by Dr. Paul Savage and colleagues. Three independent laboratories have shown
that PBS-57 activity is indistinguishable from a-galactosylceramide. The NIH Tetramer Facility provides PBS-57 ligand complexed to
CD1d monomers or tetramers.
Requests for PBS-57 ligand alone should be directed
to Dr. Savage at
Brigham Young University.
OCH
OCH, an a-galactosylceramide analogue with a truncated
side chain, stimulates Th2-biased cytokine production
in natural killer T cells. This ligand has been
shown to delay the onset of experimental autoimmune encephalomyelitis
in an animal model (Miyamoto
et al., 2001). Through a collaboration with Dr. Paul
Savage at Brigham Young University, the NIH Tetramer
Facility is providing mouse and human CD1d monomers and
tetramers loaded with OCH.
Purified OCH ligand may also be obtained for stimulation
of NK T cells in vitro or for in vivo animal
studies. (The material may not be used in humans.) OCH
is dissolved in a Tween/sucrose/histidine buffer, sterile-filtered,
placed in autoclaved vials, and lyophilized. The
resulting powder can be reconstituted in water at a final
concentration of 0.2 mg/mL.
GSL-1
Recent studies have shown that glycolipids from the Sphingomonadaceae bacterial
family are capable of stimulating natural killer T cells
through presentation of the ligands on CD1d molecules
(Kinjo
et al., 2005 and Mattner
et al., 2005) GSL-1 is structurally similar to PBS-57
and a-galactosylceramide. Through a collaboration
with Dr. Paul Savage at Brigham Young University, the
NIH Tetramer Facility is providing mouse and human CD1d
monomers and tetramers loaded with GSL-1.
Purified GSL-1 ligand may also be obtained for stimulation
of NK T cells in vitro or for in vivo animal
studies. (The material may not be used in humans.) GSL-1
is dissolved in a Tween/sucrose/histidine buffer, sterile-filtered,
placed in autoclaved vials, and lyophilized. The
resulting powder can be reconstituted in water at a final
concentration of 0.2 mg/mL.
a-C-galactosylceramide
The a-C-galactosylceramide analogue of a-galactosylceramide
is a potent stimulator of natural killer T cells and has
been shown to protect animals against certain infections
and cancers (Schmieg
et al., 2003) Through a collaboration with Dr. Richard
Franck at Hunter College of CUNY, the NIH Tetramer Facility
is offering purified synthetic a-C-galactosylceramide for in
vitro NK T cell activation.
We are not able to provide a-C-galactosylceramide-loaded
CD1d tetramers due to the instability of the reagent.
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