|
Expanded Range of Fluorophores Available
In addition to the standard PE- and APC-labeled tetramers, NIH Tetramer Facility
clients can now order reagents labeled with the following small molecule fluorophores:
Pacific Blue
Fluorescein
Alexa 488
Alexa 647
Alexa 680
Alexa 647 and Alexa 680 are more stable alternatives
to the APC label, while labeling tetramers with the green
Alexa 488 and fluorescein fluorophores allows for added
flexibility in experimental design.
| Fluorophore |
Absorption
Maxima |
Fluorescence
Emission Maxima |
Color |
| R-PE |
496, 546, 565 nm |
578 nm |
Orange |
| APC |
650 |
660 |
Far-red |
| Pacific Blue |
410 |
455 |
Blue |
| Fluorescein |
494 |
518 |
Green |
| Alexa 488 |
495 |
519 |
Green |
| Alexa 647 |
650 |
668 |
Far-red |
| Alexa 680 |
679 |
702 |
Far-red |
|
