Expanded Range of Fluorophores Available In addition to the standard PE- and APC-labeled tetramers, NIH Tetramer Facility clients can now order reagents labeled with the following small molecule fluorophores:

Fluorescein
Alexa 488
Alexa 568
Alexa 647
Alexa 680

Alexa 647 and Alexa 680 are more stable alternatives to the APC label, while labeling tetramers with the green Alexa 488 and fluorescein fluorophores allows for added flexibility in experimental design.

Please note that Alexa 568 requires a flow cytometer with a green laser.

 

Fluorophore Absorption Maxima (nm) Fluorescence Emission Maxima (nm) Color
PE 496, 546, 565 578 Orange
APC 650 660 Far-red
Fluorescein 494 518 Green
Alexa 488 495 519 Green
Alexa 568 578 603 Green
Alexa 647 650 668 Far-red
Alexa 680 679 702 Far-red