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- Facility Description
- Tetramer Science
- Available Reagents
- Excluded Tetramers
- Class I Tetramers
- Class II Tetramers
- Nonclassical MHC Molecules
- Unlisted Reagents
- Fluorophores
- Request a Reagent
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Pre-made Class II MHC tetramers
The class II MHC tetramers listed below are available as biotinylated monomers or as tetramers labeled with either PE or APC. Requesters will receive 200 micrograms of protein per order. In addition, a human CLIP peptide-containing negative control reagent will be included with all orders. (This control will automatically be included. You do not need to add it to your request.)
Please note that the reagents listed on this page have been produced by the NIH Tetramer Facility with strict quality control (including gene sequencing, affinity purification, SDS PAGE analysis, and verification of biotinylation by immunoprecipitation), but many have not been confirmed to stain or activate specific T cells.
|
MHC Allele
|
Source of Antigen
|
Peptide Sequence
|
|
I-A(b)
|
Lymphocytic choriomeningitis virus glycoprotein 66-77
|
DIYKGVYQFKSV
|
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I-A(b)
|
Lymphocytic choriomeningitis virus glycoprotein 126-140
|
TSAFNKKTFDHTLMS
|
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I-A(b)
|
Lymphocytic choriomeningitis virus glycoprotein precursor 6-20
|
TMFEALPHIIDEVIN
|
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I-A(b)
|
Mouse myelin oligodendrocyte glycoprotein 38-49
|
GWYRSPFSRVVH *
|
|
I-A(b)
|
Mouse myelin oligodendrocyte glycoprotein 38-49 (Variant)
|
GWYRSPFDRVVH *
|
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*These MOG reagents do not work on 2D2 TRCtg cells.
|
||
|
I-A(b)
|
Chicken ovalbumin 329-337
|
AAHAEINEA **
|
|
**This OVA tetramer contains a shorter version of the 323-339 epitope and results in improved staining of OT-II cells. Please click here for more details before requesting.
|
||
|
I-A(b)
|
Chicken ovalbumin 323-339
|
ISQAVHAAHAEINEAGR ***
|
|
***This Ova reagent is problematic. Very high concentrations of tetramer are required, and it still may not stain 100% of the appropriate cells. We recommend using another reagent or model system if possible.
|
||
|
I-A(b)
|
Chicken ovalbumin 258-276
|
IINFEKLTEWTSSNVMEER
|
|
I-A(b)
|
Chicken ovalbumin 265-280
|
TEWTSSNVMEERKIKV
|
|
I-A(b)
|
Human clotting factor VIII 2210-2229
|
TASSYFTNMFATWSPSKARL
|
|
I-A(b)
|
M. tuberculosis 6kDa early secretory antigenic target (ESAT-6) 1-20
|
MTEQQWNFAGIEAAASAIQG
|
|
I-A(b)
|
M. tuberculosis antigen 85B precursor 280-294
|
FQDAYNAAGGHNAVF
|
|
I-A(b)
|
Murine polyomavirus large T antigen 676-688
|
NEYLLPQTVWARF
|
|
I-A(b)
|
Murine polyomavirus major capsid protein VP1 221-235
|
GMYPVEIWHPDPAKN
|
|
I-A(b)
|
Mouse tyrosinase-related protein 1 113-126
|
CRPGWRGAACNQKI
|
|
I-A(b)
|
Simian immunodeficiency virus gag 299-311
|
DRFYKSLRAEQTD
|
|
I-A(b)
|
Friend murine leukemia virus envelope 123-141
|
EPLTSLTPRCNTAWNRLKL
|
|
I-A(b)
|
Mouse MHC class II antigen E alpha 52-68 (sequence 1)
|
ASFEAQGALANIAVDKA
|
|
I-A(b)
|
Mouse MHC class II antigen E alpha 55-68 (sequence 2)
|
EAWGALANKAVDKA
|
|
I-A(b)
|
Mouse Interstitial retinol binding protein 3 294-306
|
QTWEGSGVLPCVG
|
|
I-A(b)
|
Mouse Interstitial retinol binding protein 3 786-797
|
SYSSAVPLLCSY
|
|
I-A(b)
|
Murine hepatitis virus membrane protein M 133-147
|
TVYVRPIIEDYHTLT
|
|
I-A(b)
|
Murine hepatitis virus spike glycoprotein 358-372
|
LSSLLRYVQAESLSC
|
|
I-A(b)
|
Respiratory syncytial virus matrix protein M 209-223
|
NKGAFKYIKPQSQFI
|
|
I-A(b)
|
Human respiratory syncytial virus transcriptase protein matrix protein 2 26-39
|
NYFEWPPHALLVRQ
|
|
I-A(b)
|
Uncultured bacterium flagellin 456-475
|
DMATEMVKYSNANILSQAGQ
|
|
I-A(b)
|
Francisella tularensis 17kD lipoprotein tul4 86-99
|
RLQWQAPEGSKCHD
|
|
I-A(b)
|
Chlamydia trachomatis predicted periplasmic protein Cta1 133-152
|
KGIDPQELWVWKKGMPNWEK
|
|
I-A(b)
|
Chlamydia muridarum polymorphic membrane protein G family protein 300-315
|
SPIYVDPAAAGGQPPA
|
|
I-A(b)
|
Influenza A virus nucleocapsid protein 311-325
|
QVYSLIRPNENPAHK
|
|
I-A(b)
|
Listeria monocytogenes listeriolysin 190-201
|
NEKYAQAYPNVS
|
|
I-A(b)
|
Simian virus 40 large T antigen 529-543
|
NEYSVPKTLQARFVK
|
|
I-A(b)
|
Torpedo californica acetycholine receptor alpha chain
|
LGIWTYDGTKVSISPES
|
|
I-A(b)
|
Vaccinia virus DNA-binding core protein I1L 7-21
|
QLVFNSISARALKAY
|
|
I-A(b)
|
Vaccinia virus IMV heparin binding surface protein 269-283
|
PGVMYAFTTPLISFF
|
|
I-A(b)
|
Vaccinia virus plaque-size/host range protein B5R 46-60
|
FTCDQGYHSSDPNAV
|
|
I-A(b)
|
Murid herpesvirus 1 m09 133-147
|
GYLYIYPSAGNSFDL
|
|
I-A(b)
|
Murid herpesvirus 1 m25 409-423
|
NHLYETPISATAMVI
|
|
I-A(b)
|
Mouse Immunoglobulin gamma heavy chain (IgG) 167-186
|
PAVLQSDLYTLSSSVTVPSS
|
|
I-A(b)
|
Human Immunoglobulin gamma heavy chain (IgG) 167-187
|
PAVLQSSGLYSLSSVVTVPSS
|
|
I-A(b)
|
Mouse Immunoglobulin gamma heavy chain (IgG) 289-307
|
EEQFNSTFRSVSELPIMHQ
|
|
I-A(b)
|
Yersinia pestis F1 capsule antigen 67-84
|
LTLGGYKTGTTSTSVNFT
|
|
I-A(b)
|
Toxoplasma gondii hypothetical protein TGME49_0123000 605-619
|
AVEIHRPVPGTAPPS
|
|
I-A(b)
|
Dengue virus 2 Polyprotein 1673-1687
|
GKTKRYLPAIVREAI
|
|
I-A(b)
|
Human class II-associated invariant chain peptide 103-117
|
PVSKMRMATPLLMQA
|
|
|
|
|
|
I-A(d)
|
Influenza A virus hemagluttinin 143-155
|
HNTNGVTAACSHE
|
|
I-A(d)
|
Influenza A virus hemagluttinin 141-155 (alternative version)
|
RGHNVNGVTAACSHE
|
|
I-A(d)
|
Maltose binding protein malE 69-84
|
GYAQSGLLAEITPDKA
|
|
I-A(d)
|
Chicken ovalbumin 323-339
|
ISQAVHAAHAEINEAGR***
|
|
I-A(d)
|
Chicken ovalbumin 323-339 (Variant)
|
ISQAVHEAHIEVNESGR***
|
|
***These Ova reagents are problematic. Very high concentrations of tetramer are required, and they still may not stain 100% of the appropriate cells. We recommend using another reagent or model system if possible.
|
||
|
I-A(d)
|
Mouse H+/K+ ATPase 889-900
|
PLLCVGLRPQWE
|
|
I-A(d)
|
Mouse H+/K+ ATPase 630-641
|
PITAKAIAASVG
|
|
I-A(d)
|
Trypanosoma cruzi trans-sialidase 26-43
|
KVTERVVHSFRLPALVNV
|
|
I-A(d)
|
Leishmania activated protein kinase C receptor (LACK) 159-175
|
SPSLEHPIVVSGSWDNT
|
|
I-A(d)
|
Lymphocytic choriomeningitis virus glycoprotein 176-190
|
DAQSAQSQCRTFRGR
|
|
I-A(d)
|
Lymphocytic choriomeningitis virus glycoprotein 316-330
|
CDMLRLIDYNKAALS
|
|
I-A(d)
|
Lymphocytic choriomeningitis virus nucleoprotein 86-100
|
KNVLKVGRLSAEELM
|
|
I-A(d)
|
Lymphocytic choriomeningitis virus nucleoprotein 116-130
|
SERPQASGVYMGNLT
|
|
I-A(d)
|
Lymphocytic choriomeningitis virus nucleoprotein 176-190
|
PSLTMACMAKQSQTP
|
|
I-A(d)
|
Lymphocytic choriomeningitis virus nucleoprotein 466-480
|
SQNRKDIKLIDVEMT
|
|
I-A(d)
|
Plasmodium yoeli circumsporozoite protein precursor 57-70
|
KIYNRNIVNRLLGD
|
|
I-A(d)
|
Plasmodium yoeli circumsporozoite protein precursor 280-295
|
SYVPSAEQILEFVKQI
|
|
I-A(d)
|
Mouse aggrecan 89-103
|
ATEGQVRVNSIYQDK
|
|
I-A(d)
|
Human aggrecan 89-103
|
ATEGRVRVNSAYQDK
|
|
I-A(d)
|
Human class II-associated invariant chain peptide 103-117
|
PVSKMRMATPLLMQA
|
|
|
|
|
|
I-A(g7)
|
BDC 2.5 mimotope sequence 1
|
AHHPIWARMDA
|
|
I-A(g7)
|
BDC 2.5 mimotope sequence 2
|
RTRPLWVRME
|
|
I-A(g7)
|
BDC 15 mimotope
|
AAAAVRPLWVRMEAA
|
|
I-A(g7)
|
Chicken ovalbumin 141-160
|
CARELINSWVESQTNGIIRN
|
|
I-A(g7)
|
Rat CD23 216-231
|
DLKGRLVSIHSQKEQD
|
|
I-A(g7)
|
Rat/Mouse E25B 112-126
|
YQTIEENIKIFEEDA
|
|
I-A(g7)
|
Human glutamic acid decarboxylase 2 (65kDa) 524-543
|
SRLSKVAPVIKARMMEYGTT
|
|
I-A(g7)
|
Mouse proteolipid protein 56-70
|
DYEYLINVIHAFQYV
|
|
I-A(g7)
|
Rat/Mouse glucose-6-phosphate isomerase 282-292
|
LSIALHVGFDH
|
|
I-A(g7)
|
Mouse myelin oligodendrocyte glycoprotein 42-55
|
SPFSRVVHLYRNGK
|
|
I-A(g7)
|
Human class II-associated invariant chain peptide 103-117
|
PVSKMRMATPLLMQA
|
|
|
|
|
|
I-A(u)
|
Human class II-associated invariant chain peptide 103-117
|
PVSKMRMATPLLMQA
|
|
|
|
|
|
I-A(k)
|
Chicken egg white lysozyme 46-61
|
NTDGSTDYGILQINSR
|
|
I-A(k)
|
Human class II-associated invariant chain peptide 103-117
|
PVSKMRMATPLLMQA
|
|
|
|
|
|
I-E(d)
|
Influenza A virus hemagglutinin 127-137
|
SFERFEIFPKE
|
|
I-E(d)
|
Plasmodium chabaudi chabaudi merozoite surface protein 1 1157-1171
|
ISVLKSRLLKRKKYI
|
|
I-E(d)
|
Human class II-associated invariant chain peptide 103-117
|
PVSKMRMATPLLMQA
|
|
|
|
|
|
I-E(k)
|
Pigeon cytochrome C 89-105
|
KAERADLIAYLKQATAK
|
|
I-E(k)
|
Human class II-associated invariant chain peptide 103-117
|
PVSKMRMATPLLMQA
|
|
|
|
|
|
DRB1*0101
|
Human class II-associated invariant chain peptide 103-117
|
PVSKMRMATPLLMQA
|
|
|
|
|
|
DRB1*0301
|
Mycobacterium heat shock protein 65 3-13
|
KTIAYDEEARR
|
|
DRB1*0301
|
Human myelin proteolipid protein 97-108
|
RQIFGDYKTTIC
|
|
DRB1*0301
|
Human class II-associated invariant chain peptide 103-117
|
PVSKMRMATPLLMQA
|
|
|
|
|
|
DRB1*0401
|
Human class II-associated invariant chain peptide 103-117
|
PVSKMRMATPLLMQA
|
|
|
|
|
|
DRB1*0701
|
Human herpesvirus 5 65 kD lower matrix phosphoprotein pp65 177-191
|
EPDVYYTSAFVFPTK
|
|
DRB1*0701
|
HIV-1 Gag p24 294-313
|
FRDYVDRFYKTLRAEQASQE
|
|
DRB1*0701
|
Human class II-associated invariant chain peptide 103-117
|
PVSKMRMATPLLMQA
|
|
|
|
|
|
DRB1*1401
|
Human class II-associated invariant chain peptide 103-117
|
PVSKMRMATPLLMQA
|
|
|
|
|
|
DRB1*1501
|
Human class II-associated invariant chain peptide 103-117
|
PVSKMRMATPLLMQA
|
|
|
|
|
|
DRB5*0101
|
Human class II-associated invariant chain peptide 103-117
|
PVSKMRMATPLLMQA
|
|
|
|
|
|
DP1*0401
|
Clostridium tetani tetanus toxin 947-967
|
FNNFTVSFWLRVPKVSASHLE
|
|
DP1*0401
|
Human oxytocinase 272-284
|
KKYFAATQFEPLA
|
|
DP1*0401
|
Human immunodeficiency virus envelope glycoprotein 31-45
|
TEKLWVTVYYGVPVW
|
|
|
|
|
|
DQB1*05:01
|
Human immunodeficiency virus 1 gag protein 293-303
|
FRDYVDRFYKT
|
|
DQB1*05:01
|
Human Fc fragment of IgE (Rc for CD23) 104-122
|
SQDLELSWNLNGLQADLSS
|
|
|
|
|
|
Patr-DRB1*0701
|
Hepatitis C virus NS4 1790-1799
|
LMAFTAAVTS
|
|
Patr-DRB1*0701
|
Human class II-associated invariant chain peptide 103-117
|
PVSKMRMATPLLMQA
|
|
|
|
|
|
Patr-DRB1*1001
|
Hepatitis C virus NS3 1248-1258
|
GYKVLVLNPSV
|
|
Patr-DRB1*1001
|
Human class II-associated invariant chain peptide 103-117
|
PVSKMRMATPLLMQA
|
|
|
|
|
|
Patr-DRB5*0102
|
Hepatitis C virus envelope protein 2 354-369
|
LPCSFTTLPALSTGLI
|
|
Patr-DRB5*0102
|
Hepatitis C virus NS4b 1761-1775
|
WAKHMWNFISGIQYL
|
|
Patr-DRB5*0102
|
Hepatitis C virus NS3 1432-1445
|
VVVVSTDALMTGFT
|
|
Patr-DRB5*0102
|
Human class II-associated invariant chain peptide 103-117
|
PVSKMRMATPLLMQA
|
*This MOG reagent does not work on 2D2 TRCtg cells.
**This OVA tetramer contains a shorter version of the 323-339 epitope and results in improved staining of OT-II cells. Please click here for more details before requesting.
***This Ova reagent is problematic. Very high concentrations of tetramer are required, and it still may not stain 100% of the appropriate cells. We recommend using another reagent or model system if possible.
Disclaimer: Please note that the reagents listed on this page have been produced by the NIH Tetramer Facility with strict quality control (including gene sequencing, affinity purification, SDS PAGE analysis, and verification of biotinylation by immunoprecipitation), but many have not been confirmed to stain or activate specific T cells.